Heterologous expression of Aspen PTM3, a MADS box gene in cotton.
Identifieur interne : 002E42 ( Main/Exploration ); précédent : 002E41; suivant : 002E43Heterologous expression of Aspen PTM3, a MADS box gene in cotton.
Auteurs : E. Ramachandran [Inde] ; Shyamal K. Bhattacharya ; S A John ; P S Bhattacharya ; Gerard AbrahamSource :
- Journal of biotechnology [ 1873-4863 ] ; 2011.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (métabolisme), Amorces ADN (génétique), Analyse de variance (MeSH), Glucuronidase (MeSH), Gossypium (métabolisme), Populus (génétique), Protéines à domaine MADS (génétique), Protéines à domaine MADS (métabolisme), Réaction de polymérisation en chaîne (MeSH), Sélection (méthodes), Technique de Southern (MeSH), Technique de Western (MeSH), Transformation génétique (MeSH).
- MESH :
- génétique : Amorces ADN, Populus, Protéines à domaine MADS.
- métabolisme : Agrobacterium tumefaciens, Gossypium, Protéines à domaine MADS.
- méthodes : Sélection.
- Analyse de variance, Glucuronidase, Réaction de polymérisation en chaîne, Technique de Southern, Technique de Western, Transformation génétique.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (metabolism), Analysis of Variance (MeSH), Blotting, Southern (MeSH), Blotting, Western (MeSH), Breeding (methods), DNA Primers (genetics), Glucuronidase (MeSH), Gossypium (metabolism), MADS Domain Proteins (genetics), MADS Domain Proteins (metabolism), Polymerase Chain Reaction (MeSH), Populus (genetics), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : DNA Primers, MADS Domain Proteins.
- genetics : Populus.
- metabolism : Agrobacterium tumefaciens, Gossypium, MADS Domain Proteins.
- methods : Breeding.
- Analysis of Variance, Blotting, Southern, Blotting, Western, Glucuronidase, Polymerase Chain Reaction, Transformation, Genetic.
Abstract
The PTM3 gene of Aspen was ectopically expressed in cotton to explore the opportunity to introduce desirable agronomic traits with the potential to improve yield and modify the duration of the parent cotton variety. Sixty-seven transgenic cotton lines expressing Aspen PTM3 (MADS box) gene were developed. The transgenic cotton lines expressing PTM3 gene showed earliness of 4-15 days variations in flowering and maturity. The transgenic lines were confirmed by kanamycin leaf paint assay, GUS assay and PCR. Among 67 transgenic lines, the event-10 showed profuse branching, event-24 showed abnormal growth and the remaining events exhibited single erect phenotype. In addition, the event-24 produced no flower and this might be due to the positional effect of PTM3 gene integration. Southern blot analysis performed for event-10, 24 and 48 showed distinct single copy integrations of PTM3 gene cassette. GUS assay performed using various plant parts of event-10 showed constitutive expression of the transgene. In view of cotton breeding, among all the events, the event-10 was found to be phenotypically significant with earliness of 12 days in flowering and 15 days in maturity and yield enhancement of 27%. In addition, the event-10 showed no square dropping and allowed the plants to bear more number of bolls. Based on these results, event-10 was chosen to carry out the inheritance study of expressed characters in the progeny.
DOI: 10.1016/j.jbiotec.2011.06.015
PubMed: 21723337
Affiliations:
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Ramachandran</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, SHIATS (Allahabad Agricultural Institute-DU), Allahabad, India.ramachandrane1@rediffmail.com</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Inde</country><wicri:regionArea>Department of Biological Sciences, SHIATS (Allahabad Agricultural Institute-DU), Allahabad</wicri:regionArea><wicri:noRegion>Allahabad</wicri:noRegion></affiliation></author><author><name sortKey="Bhattacharya, Shyamal K" sort="Bhattacharya, Shyamal K" uniqKey="Bhattacharya S" first="Shyamal K" last="Bhattacharya">Shyamal K. Bhattacharya</name></author><author><name sortKey="John, S A" sort="John, S A" uniqKey="John S" first="S A" last="John">S A John</name></author><author><name sortKey="Bhattacharya, P S" sort="Bhattacharya, P S" uniqKey="Bhattacharya P" first="P S" last="Bhattacharya">P S Bhattacharya</name></author><author><name sortKey="Abraham, Gerard" sort="Abraham, Gerard" uniqKey="Abraham G" first="Gerard" last="Abraham">Gerard Abraham</name></author></analytic><series><title level="j">Journal of biotechnology</title><idno type="eISSN">1873-4863</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (metabolism)</term><term>Analysis of Variance (MeSH)</term><term>Blotting, Southern (MeSH)</term><term>Blotting, Western (MeSH)</term><term>Breeding (methods)</term><term>DNA Primers (genetics)</term><term>Glucuronidase (MeSH)</term><term>Gossypium (metabolism)</term><term>MADS Domain Proteins (genetics)</term><term>MADS Domain Proteins (metabolism)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Populus (genetics)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (métabolisme)</term><term>Amorces ADN (génétique)</term><term>Analyse de variance (MeSH)</term><term>Glucuronidase (MeSH)</term><term>Gossypium (métabolisme)</term><term>Populus (génétique)</term><term>Protéines à domaine MADS (génétique)</term><term>Protéines à domaine MADS (métabolisme)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Sélection (méthodes)</term><term>Technique de Southern (MeSH)</term><term>Technique de Western (MeSH)</term><term>Transformation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term><term>MADS Domain Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term><term>Populus</term><term>Protéines à domaine MADS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Gossypium</term><term>MADS Domain Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Breeding</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Gossypium</term><term>Protéines à domaine MADS</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Sélection</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term><term>Blotting, Southern</term><term>Blotting, Western</term><term>Glucuronidase</term><term>Polymerase Chain Reaction</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de variance</term><term>Glucuronidase</term><term>Réaction de polymérisation en chaîne</term><term>Technique de Southern</term><term>Technique de Western</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The PTM3 gene of Aspen was ectopically expressed in cotton to explore the opportunity to introduce desirable agronomic traits with the potential to improve yield and modify the duration of the parent cotton variety. Sixty-seven transgenic cotton lines expressing Aspen PTM3 (MADS box) gene were developed. The transgenic cotton lines expressing PTM3 gene showed earliness of 4-15 days variations in flowering and maturity. The transgenic lines were confirmed by kanamycin leaf paint assay, GUS assay and PCR. Among 67 transgenic lines, the event-10 showed profuse branching, event-24 showed abnormal growth and the remaining events exhibited single erect phenotype. In addition, the event-24 produced no flower and this might be due to the positional effect of PTM3 gene integration. Southern blot analysis performed for event-10, 24 and 48 showed distinct single copy integrations of PTM3 gene cassette. GUS assay performed using various plant parts of event-10 showed constitutive expression of the transgene. In view of cotton breeding, among all the events, the event-10 was found to be phenotypically significant with earliness of 12 days in flowering and 15 days in maturity and yield enhancement of 27%. In addition, the event-10 showed no square dropping and allowed the plants to bear more number of bolls. Based on these results, event-10 was chosen to carry out the inheritance study of expressed characters in the progeny.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21723337</PMID><DateCompleted><Year>2011</Year><Month>12</Month><Day>12</Day></DateCompleted><DateRevised><Year>2011</Year><Month>08</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4863</ISSN><JournalIssue CitedMedium="Internet"><Volume>155</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Sep</Month><Day>10</Day></PubDate></JournalIssue><Title>Journal of biotechnology</Title><ISOAbbreviation>J Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Heterologous expression of Aspen PTM3, a MADS box gene in cotton.</ArticleTitle><Pagination><MedlinePgn>140-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiotec.2011.06.015</ELocationID><Abstract><AbstractText>The PTM3 gene of Aspen was ectopically expressed in cotton to explore the opportunity to introduce desirable agronomic traits with the potential to improve yield and modify the duration of the parent cotton variety. Sixty-seven transgenic cotton lines expressing Aspen PTM3 (MADS box) gene were developed. The transgenic cotton lines expressing PTM3 gene showed earliness of 4-15 days variations in flowering and maturity. The transgenic lines were confirmed by kanamycin leaf paint assay, GUS assay and PCR. Among 67 transgenic lines, the event-10 showed profuse branching, event-24 showed abnormal growth and the remaining events exhibited single erect phenotype. In addition, the event-24 produced no flower and this might be due to the positional effect of PTM3 gene integration. Southern blot analysis performed for event-10, 24 and 48 showed distinct single copy integrations of PTM3 gene cassette. GUS assay performed using various plant parts of event-10 showed constitutive expression of the transgene. In view of cotton breeding, among all the events, the event-10 was found to be phenotypically significant with earliness of 12 days in flowering and 15 days in maturity and yield enhancement of 27%. In addition, the event-10 showed no square dropping and allowed the plants to bear more number of bolls. Based on these results, event-10 was chosen to carry out the inheritance study of expressed characters in the progeny.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ramachandran</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, SHIATS (Allahabad Agricultural Institute-DU), Allahabad, India.ramachandrane1@rediffmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bhattacharya</LastName><ForeName>Shyamal K</ForeName><Initials>SK</Initials></Author><Author ValidYN="Y"><LastName>John</LastName><ForeName>S A</ForeName><Initials>SA</Initials></Author><Author ValidYN="Y"><LastName>Bhattacharya</LastName><ForeName>P S</ForeName><Initials>PS</Initials></Author><Author ValidYN="Y"><LastName>Abraham</LastName><ForeName>Gerard</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>06</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Biotechnol</MedlineTA><NlmUniqueID>8411927</NlmUniqueID><ISSNLinking>0168-1656</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D026161">MADS Domain Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000704" MajorTopicYN="N">Analysis of Variance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001947" MajorTopicYN="N">Breeding</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003368" MajorTopicYN="N">Gossypium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D026161" MajorTopicYN="N">MADS Domain Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>01</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>06</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>06</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>7</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>7</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>12</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21723337</ArticleId><ArticleId IdType="pii">S0168-1656(11)00314-2</ArticleId><ArticleId IdType="doi">10.1016/j.jbiotec.2011.06.015</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Abraham, Gerard" sort="Abraham, Gerard" uniqKey="Abraham G" first="Gerard" last="Abraham">Gerard Abraham</name><name sortKey="Bhattacharya, P S" sort="Bhattacharya, P S" uniqKey="Bhattacharya P" first="P S" last="Bhattacharya">P S Bhattacharya</name><name sortKey="Bhattacharya, Shyamal K" sort="Bhattacharya, Shyamal K" uniqKey="Bhattacharya S" first="Shyamal K" last="Bhattacharya">Shyamal K. Bhattacharya</name><name sortKey="John, S A" sort="John, S A" uniqKey="John S" first="S A" last="John">S A John</name></noCountry><country name="Inde"><noRegion><name sortKey="Ramachandran, E" sort="Ramachandran, E" uniqKey="Ramachandran E" first="E" last="Ramachandran">E. Ramachandran</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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